MTT Assay for Cell Viability

The MTT calorimetric assay determines the ability of viable cells to convert a soluble tetrazolium salt [3–-2

MTT is a pale yellow substrate and formazan a violet dye This cell reduction reaction involves the NADH NADPH pyridine nucleotide cofactors and is only

mtt The MTT assay was the first widely accepted method that replaced the radioactive tritiated thymidine incorporation assay to measure cell proliferation

mtt The MTT reagent alone results in very low background absorbance values in the absence of cells on Alvetex Scaffold It is recommended that for each cell type